Chapter 1 creation (pages 1–4): P. N. R. Usherwood
Chapter 2 Chemical Signalling within the Insect apprehensive method (pages 5–11): John G. Hildebrand
Chapter three Acetylcholine Receptors of pointed out Insect Neurons (pages 12–31): Ian D. Harrow, Jonathan A. David and David B. Sattelle
Chapter four homes of Dopamine Receptors at a Neuroglandular Synapse (pages 32–47): C. R. apartment and B. L. Ginsborg
Chapter five homes of Modulatory Octopamine Receptors within the Locust (pages 48–69): Peter D. Evans
Chapter 6 houses of Postsynaptic Channels Activated through Glutamate and GABA in Locust Muscle Fibres (pages 70–87): S. G. Cull?Candy
Chapter 7 Neurosecretory Peptides and Biogenic Amines (pages 88–108): W. Mordue
Chapter eight Isolation of Receptors from the principal fearful approach (pages 109–117): E. A. Barnard
Chapter nine Pharmacological features of a Putative Nicotinic Acetylcholine Receptor from Musca dornestica (pages 118–136): Christine S. March, Kenneth J. Cattell and John F. Donnellan
Chapter 10 Binding of Acetylcholine Receptor/Channel Probes to Housefly Head Membranes (pages 137–152): Amira T. Eldefrawi, Nader Shaker and Mohyee E. Eldefrawi
Chapter eleven Subcellular Fractionation of Invertebrate anxious Tissue (pages 153–157): Richard W. Olsen
Chapter 12 homes of Synaptosomes from the primary frightened approach of bugs (pages 158–175): Heinz Breer
Chapter thirteen Binding and Uptake of Glutamate and ??Aminobutyric Acid in Membrane Fractions from Locust Muscle (pages 176–196): Patricia A. Briley, Marie T. Filbin, George G. Lunt and John F. Donnellan
Chapter 14 Genetic methods to Insect Neurochemistry (pages 199–206): Yadin Dudai
Chapter 15 identity of a Drosophila melanogaster Mutant that has effects on the Saxi Toxin Receptor of the Volt Age?Sensitive Sodium Channel (pages 207–220): Linda M. corridor, Susan D. Wilson, Jane Gitschier, Nancy Martinez and Gary R. Strichartz
Chapter sixteen Genetic and Immunological experiences of the frightened approach of Drosophila melanogaster (pages 221–239): Yuh Nung Jan and Lily Yeh Jan
Chapter 17 Activation of Ion Channels in Locust Muscle by way of Amino Acids (pages 240–259): ok. A. F. Gration
Chapter 18 Receptor Mechanisms Mediating the motion of 5?Hydroxytryptamine (pages 260–274): Michael J. Berridge and John P. Heslop
Chapter 19 Solitary Wasp Venoms and pollutants as instruments for the learn of Neuromuscular Transmission in bugs (pages 275–290): Tom Piek
Chapter 20 pesticides as Probes for the learn of Ionic Channels in Nerve Membranes (pages 291–306): Toshio Narahashi
Chapter 21 evaluate of the Symposium (pages 307–317): P. N. R. Usherwood
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Additional info for Ciba Foundation Symposium 88 - Neuropharmacology of Insects
5 mM-EGTA on secretory responses of salivary gland cells to dopamine. The dopamine (DA) was applied in the bathing solution at a concentration of 1 pM for the periods indicated by the horizontal bars. dopamine is relatively insensitive to calcium deprivation (Fig. 3). However, during a sequence of dopamine applications the amplitudes of the responses fall and, moreover, during an individual response the peak rate is not so well maintained as it is in the presence of extracellular calcium. It might be expected that a long stimulation by dopamine in a ‘calcium-free’ solution would yield a response that would gradually decline almost to zero.
0 x 10-3 M. At higher concentrations a blocking action was noted (David & Sattelle 1982). ACETYLCHOLINE RECEPTORS 23 Thus of the three molecular probes used so far in binding studies to characterize putative cholinergic receptors in insects, a-BGTX was the most effective on the ACh receptors of the cell body membrane of Df. Quinuclidinyl benzilate was three orders of magnitude less effective and decamethonium was five orders of magnitude less effective than a-BGTX. As noted for GI2, nicotinic cholinergic receptors are clearly present on the extrasynaptic (cell body) membrane of Df.
However, the crucial evidence is lacking that dopamine is released by nerve stimulation in sufficient quantity to stimulate the gland cells. The neuroglandular synapses in the acini are not distinguished by pre- or postsynaptic membrane thickenings. Transmitter released from varicosities on the acinar surface would have to travel about 1 pm to the nearest gland cell membrane, whereas the length of the diffusion path for the release sites on the nerve terminals is about 20 nm. The location of the receptors in the peripheral and central cells is uncertain but we assume that they occur in their basal membranes.