Cumulative Subject Index Volume 31-52 by Leslie Wilson

By Leslie Wilson

Severely acclaimed for greater than 25 years, the equipment in telephone Biology sequence offers an fundamental instrument for the researcher. every one quantity is thoroughly edited by means of specialists to comprise state of the art studies and step by step protocols. recommendations are defined thoroughly in order that tools are made obtainable to clients.

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50, 127 phenyl-sepharose CL4B, 47,344-345 procion red column, 47,469 receptor-mediated endocytosis and, 32,309 replica plating and, 32, 405, 406 RNA-binding protein isolation, 44, 195-196 secretory path in yeast and, 32, 15, 28 superose-12 gel filtration, 47,345-346 synexin and, 31,210,211 template-active nucleosomes and, see Template-active nucleosomes thin-layer, see Thin-layer chromatography vesicular stomatitis virus mutants and, 32, 106, 107 yeast organelle fractionation and, 31,350 Chromatophores, 31,23 cytoskeleton, 31, 7, 8 dermal, 31, 6, 7 dispersion, 31, 13, 18-22 methods choice of species, 31,s isolation from dermis, 31, 12, 13 lysis, 31, 13-17 pigment transport, 31, 16, 18, 19 removal of dermis, 31,12 removal of epidermis, 31,9-12 removal of scales, 31,9 organelle transport, 31, 4-6, 19 ATP, 31,20 potential motors, 31, 22 protein, 31,21,22 regulation, 31, 20, 21 properties.

552 cytochrome P-450 and, 33,73 DNA sequence localization and, 35,4, 6, 12 electron microscopic in siru hybridization and, 35,110,121 environmental health and, 33,585, 589, 597, 602,603,606 ER-to-Golgi transport and, 31,93 47 Subject Index fingerprinted, 48,536,539 glycoinositol phospholipid anchors and, 32, 240 glycoprotein study and, 32, 123, 136 homozygous for recessive lethal mutation, generating and analyzing, 44,58-64 by chromosome loss, 44,60-61 germline, 44,63-64 by injection of cells or nuclei, 44, 63 by mitotic recombination, 44, 61-62 immunoisolation and, 31, 275, 289 intracellular vesicle fusion and, 31, 187 macromolecule delivery into cells, 31, 159 markers dilution by cell proliferation, 36,295 fluorescent dextran, 36,285-295 novel, for analyzing clones in developing or internal tissues, 44,669-670 meiotic chromosome preparation and, 35, 194-195 mitotic, in making mosaic muscles, 44,248 monoclonal antibody libraries and, 35, 230, 236,239,242,244 mRNA localization and, 35,50-51 mutations affecting cell division and, 35, 544-545,581 Drosophila genes, 35,567-577 investigations of Drosophila genes, 35,578 strategies for isolation, 35,556 mutations affecting chromosomal proteins and, 35,489-491,496,514 mutations affecting nuclear organization and, 35,528,537-539 nucleosome transcription and, 35,423 physical mapping, 48,534-536 plasma membrane and, 31,55 polyamine buffer and, 33,377 polymeric immunoglobulin receptor and, 32, 330,334 polytene chromosomes of Drosophila and, 35,222,224 position-effect variegation and, 35, 592, 624 dosage effects, 35, 610 Su(var) loci analyses, 35,612-617 positive, identification in interaction trap, 49, 412-413 protein-DNA interactions in vivo and, 35, 384,408,410 replica plating and, 32,388 CHO cells, 32, 389, 391 mutants, 32,400,401, 403,404,407, 409, 414,417,419 transfer to polyester, 32, 395 rescue mutant assay, 48,470-472 sensitive to cell death, 46, 101 subclones autoantibodies and, 35, 169 meiotic chromosome preparation and, 35, 194 supravital cell staining and, 33, 90 surface antigens and, 32,41,42 uniformity, 44,173-174 vesicular stomatitis virus mutants and, 32986 Cloning, see also Complementary DNA; Subcloning adhesin as translational fusion, 45,186-187 cDNAs, 49,423 cell death genes by differential display, 46, 112-119 by gene expression screen, 46,122-131 by pluslminus screening, 46, 120-122 cells, 44,165-166 in cellular adhesion analysis, 44,688-690 by complementation of Toxoplasma gondii, 45,57-58 DNA, in extracellular protein characterization, 44,318-319 expression cloning in oocytes, 50,528-529 gene identified by mutation in “mutation first‘‘ strategy, 44,41-43 genes, 41,522-523 plant genes, by complementation, 49, 417-428 positional, 46, 414; 48, 47 in mouse, resources for, 46,428-429 site selection for adenovirus design, 52, 235-236 in stable transformations, 44, 176 transcription factors, 52,468 virus-producing cells, 52,219-221 CMPase, lectin-gold-induced density perturbation and, 31,257 CMV, see Cytomegalovirus c-myb gene, template-active nucleosomes and, 35,331 c-myc gene epitope tag vector construction, 49,144-146 overexpression, 46, 159-160 template-active nucleosomes and, 35, 319-322,330-331 Coated pits function, control by cytoplasmic pH, 32, 365-367 EM, 32,376-380 Subject Index 48 endocytosis ricin.

312 isolation methods, 35,295-296,299, 301-302 mapping nucleosome position, 35, 304-306,308-309,312 Chromatin assembly chromatin gap in, 35,597 extracts for, 44,207-222 preparation, 44,209-212 from postblastoderm embryos, 44, 211-212 from preblastoderm embryos, 44, 209-21 1 incorporation of exogenous histone H1 into, 44,213 nucleosome organization at specific sites in, 44,219-221 position-effect variegation and, 35, 600, 612, 615, 617 replication coupled with, 44,214 using postblastoderm embryo extracts and exogenous histones, 44,213-214 using preblastoderm embryo extracts, 44, 212-213 Chromatin footprinting, see Protein-DNA interactions in vivo Chromatofocusing, synexin and, 31,211,212 Chromatography affinity techniques, see Affinity chromatography coated-vesicle adaptors and, 31, 232, 234, 236 DEAE column, 47,470 extracellular matrix protein isolation from cell cultures, 44,310-312 flow cytometry and, 31,314 fluorescence microscopy and, 31,361 fluorescent dextran, 36,291 gel filtration, 47,451 gel filtration and anion exchange, 50, 152-153 glycoinositol phospholipid anchors and, 32, 236,244-246,249,252 glycoprotein oligosaccharide remodeling and, 32,342, 346 glycoprotein oligosaccharides and, 32, 160- 168 protein glycosylation, 32, 147, 148, 150 radiolabeling, 32,152, 155, 168-172, 175 glycoprotein study and, 32, 120, 121, 128 glycosaminoglycansand, 32,212, 216, 218-220, 224 high-performance liquid, see Highperformance liquid chromatography hydrophobic interaction, 47, 133 Subject Index 43 immunoaffinity, 44,268-269,271-272 in virro nuclear protein import and, 35, 476-477 ion-exchange, see Ion-exchange chromatography lipocortin I and, 31,222 molecular sieve, in isolation of extracellular matrix proteins from cell cultures, 44, 310-312 Mono Q anion-exchange,47,319-320, 346-348,357,489 Ni affinity, protein purification, 50,465-466 nucleosome transcription and affinity, 35, 423 hydroxylapatite, 35,428-430 organomercurial.

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