By Robert G. White
This can be a booklet that I wrote for myself. It was once began sixteen years in the past while my tasks started to comprise the origina tion of ultraviolet spectrophotometric checking out tools for items of in terest to my corporation. Painful and wasteful stories of rediscovering somebody else's ana lytical strategies quickly ended in my maintaining notebooks and card records of released UV tools. again and again considering, those documents have enabled me to prevent carrying out long experiments or making tedious literature searches. whilst i made a decision to percentage them with others, I enormously elevated their scope to incorporate scientific, biochemical, and pharmacological research, in addition to different subject matters now not commonly a part of both my accountability or my in terests. This quantity contains greater than 1600 references to analyses accom plished utilizing UV absorption measurements, prepared alphabetically via senior writer. The ebook is compound-oriented; that's, it offers with fabrics, and such a lot papers dealing basically with instrumentation and strategies were excluded. a few of these goods in basic terms point out the topic taken care of. Others are abstracts containing adequate element to allow use of the tactic with no consulting the unique article. This e-book is meant, notwithstanding, no longer only to notify the reader and to provide him quick entry to the avail capable literature, yet to stimulate creativity through a short overview of the ap proaches others have taken to an issue just like his personal.
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It is a ebook that I wrote for myself. It used to be begun sixteen years in the past whilst my tasks started to comprise the origina tion of ultraviolet spectrophotometric trying out equipment for items of in terest to my corporation. Painful and wasteful stories of rediscovering anyone else's ana lytical systems quickly resulted in my protecting notebooks and card documents of released UV equipment.
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Extra info for Handbook of Ultraviolet Methods
Recovery is 103 ± 5%. 150. Bland, D. E . , Stamp, C . , AJAC-6-353-55. Vanillin, acetovanillone (4-hydroxy-3-methoxyacetophenone, and s yr ing aldehyde are determined in lignin oxidation products by chromatography on paper impregnated with sodium tetraborate, elution with CH3OH, and measurement of A at 300 nm. 151. Blatz, L. A . , ANCH-33-249-61. Cerium can be determined in the range 5-12 x 10~6 Mby measuring A at 320, 340, and 360 nm in 10-cm cells before and after reducing Ce4+to Ce3+with dil H 2 O 2 .
Chatterjee, R . , Guha, M. , JAPM-40-233-51. Cites absorptivity of vitamin A acetate thrice re crystallized from CH3OH in the following solvents: EtOH; isopropyl alcohol; and cyclohexane. 265. Chen, P. , ANCH-31-292-59. A4-3-Ketosteroids can be determined by UV after forming the hydrazone with salicoyl hydrazide. The technique can be applied to urinary aldosterone after preliminary purification by paper chromatography. Absorbance is read at 270, 295, and 320 nm after elution with 50% EtOH. Allen's correction is used: y-Aldosterone is proportional to A295 nm -O.
DRST-243-147-55. , pyrobutamine diphosphate and methapyrilene hydrochloride, can be analyzed by a two-component UV method. 155. , CKFR-11-210-62. Determination of sarcolysine, which is (±)-3-[£-di-(2-chloroethyl)aminophenyl]alanine chloride: Dissolve 50 mg in CH3OH and dilute to 250 ml; further dilute 5 ml to 100 ml and read A at 262 nm. If tablets, crush and mix ten of them and weigh an amount equivalent to ca 50 mg sarcolysine. Shake 15 min with 100 ml CH3OH, dilute to 250 ml, and let settle.